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Product Name
SPAG11A antibody
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Description
SPAG11A Rabbit Polyclonal antibody. Positive WB detected in human testis tissue, human brain tissue. Positive IHC detected in human testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue. Observed molecular weight by Western-blot: 20kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
EP2 antibody; HE2 antibody; Protein EP2 antibody; SPAG11A antibody; Sperm antigen HE2 antibody; sperm assOCiated antigen 11A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPAG11A recombinant protein (Accession Number: BC058833). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:115529(SPAG11A antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human testis using Catalog No:115529(SPAG11A antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:115529(SPAG11A antibody) at dilution of 1:50 (under 40x lens)
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Background
Sperm binding proteins and their C-terminal peptides of the Sperm Associated Antigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671).SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing.This antibody is specific to SPAG11.
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References
- Pujianto DA, Loanda E, Sari P, Midoen YH, Soeharso P. Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner. Reproductive biology and endocrinology : RB&E. 11:59. 2013.
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