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Product Name
SOX2 antibody
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Description
SOX2 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 37-40kd
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
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Alternative names
ANOP3 antibody; MCOPS3 antibody; SOX2 antibody; Transcription factor SOX 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SOX2 recombinant protein (Accession Number: NM_003106). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115514(SOX2 antibody) at dilution of 1:600
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115514(SOX2 Antibody) at dilution of 1:5000
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Background
Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. Affinity purified rabbit anti-Sox2 antibody can be used to demonstrate pluripotency of ES and iPS cells. This antibody is a rabbit polyclonal antibody raised against full length SOX2 of human origin.
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References
- Wang Z, Dao R, Bao L. Epigenetic reprogramming of human lung cancer cells with the extract of bovine parthenogenetic oocytes. Journal of cellular and molecular medicine. 18(9):1807-15. 2014.
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