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Product Name
SOX13 antibody
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Description
SOX13 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, SH-SY5Y cells. Positive IP detected in SH-SY5Y cells. Positive IHC detected in human kidney tissue. Positive IF detected in Hela cells, HepG2 cells. Observed molecular weight by Western-blot: 100kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ICA12 antibody; Islet cell antigen 12 antibody; Sox 13 antibody; SOX13 antibody; Transcription factor SOX 13 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SOX13 recombinant protein (Accession Number: NM_005686). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115511(SOX13 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115511(SOX13 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115511(SOX13 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using SOX13 antibody Catalog No:115511 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-SOX13 (IP:Catalog No:115511, 4ug; Detection:Catalog No:115511 1:500) with SH-SY5Y cells lysate 1200ug.
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Background
SRY-BOX 13(SOX13), identified a sequence homologous more than 60% to the SRY HMG box ,with a centrally located HMG box and an N-terminal leucine zipper motif with a neighboring glutamine stretch called a Q box. SOX13 binds to the consensus HMG-box motif. It's an IDDM-specific human autoantigen, and a gamma-delta-specific gene in the immune system. It can modulates Wnt/TCF activity. This is a rabbit polyclonal raised against part of N-terminal chain of SOX13 of human origin.
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References
- Daigle M, Roumaud P, Martin LJ. Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development. Molecular and cellular biochemistry. 407(1-2):209-21. 2015.
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