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Product Name
SOS1 Polyclonal Antibody
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Description
Polyclonal antibody to SOS1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SOS1 antibody; GF1 antibody; GGF1 antibody; GINGF antibody; HGF antibody; NS4 antibody; son of sevenless homolog 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1034-1333 of human SOS1 (NP_005624.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - SOS1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SOS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - SOS1 Polyclonal Antibody
Immunofluorescence analysis of rat brain using SOS1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - SOS1 Polyclonal Antibody
Immunofluorescence analysis of rat heart using SOS1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Promotes the exchange of Ras-bound GDP by GTP. Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF. Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"