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Product Name
Human SHH / Sonic hedgehog (aa 198-462, His Tag) recombinant protein
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Description
Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity).
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Protein name
Sonic hedgehog protein
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Protein short names
HPE3; HXL3; TPT; SONIC HEDGEHOG HOMOLOG; HX; HLP3; HHG1; 9530036O11RIK; HHG-1; SHORT DIGITS; TPTPS; SONIC HEDGEHOG PROTEIN; MCOPCB5; SMMCI; M100081; HEMIMELIC EXTRA TOES; DSH
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Uniprot ID
Q15465
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Gene Name
SHH
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the amino acid sequence (Cys 198-Ser 462) of human SHH (Q15465), that is Sonic hedgehog protein C-product, was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human SHH( aa 198-462) consists of 276 amino acids and has a predicted molecular mass of 29 kDa. The apparent molecular mass of the protein is approximately 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse fibroblasts.
The ED50 for this effect is 12-24 μg/mL. -
Validations
Human SHH / Sonic hedgehog Protein (aa 198-462, His Tag) SDS-PAGE
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