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Product Name
SODD/BAG4 antibody
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Description
SODD/BAG4 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive WB detected in K-562 cells, MCF7 cells. Observed molecular weight by Western-blot: 60 kDa, 66-70 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
BAG 4 antibody; BAG4 antibody; Silencer of death domains antibody; SODD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SODD/BAG4 recombinant protein (Accession Number: NM_004874). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:115495(BAG4 antibody) at dilution of 1:1000
Immunofluorescent analysis of MCF-7 cells using Catalog No:115495(BAG4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
BAG4, also known as SODD (the silencer of death domain), belongs to the BAG family of anti-apoptotic proteins. BAG4 binds to the tumor necrosis factor receptor 1 (TNFR1) and block signaling for subsequent activation of apoptosis. This antibody detects 66-70 kDa full-length BAG4 protein and 45 kDa, 60 kDa C-terminal fragments, which was also observed by Hedda et al. (12706861).
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