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Product Name
SOD3 antibody
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Description
SOD3 Rabbit Polyclonal antibody. Positive IP detected in human placenta tissue. Positive WB detected in human heart tissue, human placenta tissue. Observed molecular weight by Western-blot: 25-33 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
EC SOD antibody; Extracellular superoxide dismutase antibody; SOD3 antibody; Superoxide Dismutase 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SOD3 recombinant protein (Accession Number: NM_003102). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:115494(SOD3 antibody) at dilution of 1:1000
IP Result of anti-SOD3 (IP:Catalog No:115494, 4ug; Detection:Catalog No:115494 1:1000) with human placenta tissue lysate 2800ug.
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Background
SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2. SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa.
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