SOCS4 Polyclonal Antibody

Cat.#: 165656

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Product Information

  • Product Name
    SOCS4 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to SOCS4
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse
  • Alternative names
    SOCS4 antibody; SOCS7 antibody; suppressor of cytokine signaling 4 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SOCS4 (NP_543143.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:200
  • Validations

    Western blot - SOCS4 Polyclonal Antibody

    Western blot - SOCS4 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using SOCS4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunofluorescence - SOCS4 Polyclonal Antibody

    Immunofluorescence - SOCS4 Polyclonal Antibody

    Immunofluorescence analysis of U2OS cells using SOCS4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. Substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits EGF signaling by mediating the degradation of the Tyr-phosphorylated EGF receptor/EGFR.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"