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Product Name
SOAT1 Polyclonal Antibody
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Description
Polyclonal antibody to SOAT1
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
SOAT1 antibody; ACACT antibody; ACAT antibody; ACAT-1 antibody; ACAT1 antibody; SOAT antibody; STAT antibody; sterol O-acyltransferase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SOAT1 (NP_003092.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - SOAT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SOAT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"