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Product Name
SNX9 antibody
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Description
SNX9 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IP detected in mouse heart tissue. Positive WB detected in HeLa cells, human heart tissue, mouse skeletal muscle tissue, rat heart tissue. Observed molecular weight by Western-blot: 70-78 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MST155 antibody; MSTP155 antibody; Protein SDP1 antibody; SDP1 antibody; SH3PX1 antibody; SH3PXD3A antibody; SNX9 antibody; sorting nexin 9 antibody; WISP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SNX9 recombinant protein (Accession Number: NM_016224). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115486(SNX9 antibody) at dilution of 1:500
IP Result of anti-SNX9 (IP:Catalog No:115486, 3ug; Detection:Catalog No:115486 1:1000) with mouse heart tissue lysate 9500ug.
Immunofluorescent analysis of HeLa cells using Catalog No:115486(SNX9 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
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Background
Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).
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References
- Cao H, Yin X, Cao Y. FCHSD1 and FCHSD2 are expressed in hair cell stereocilia and cuticular plate and regulate actin polymerization in vitro. PloS one. 8(2):e56516. 2013.
- Li Chew C, Lunardi A, Gulluni F. In Vivo Role of INPP4B in Tumor and Metastasis Suppression through Regulation of PI3K-AKT Signaling at Endosomes. Cancer discovery. 5(7):740-51. 2015.
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