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Product Name
SNX6 antibody
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Description
SNX6 Rabbit Polyclonal antibody. Positive FC detected in RAW 264.7 cells. Positive IHC detected in human heart tissue, human prostate cancer tissue. Positive WB detected in RAW 264.7 cells, HepG2 cells, human placenta tissue, mouse brain tissue. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, IHC, WB, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MSTP010 antibody; SNX6 antibody; sorting nexin 6 antibody; TFAF2 antibody; TRAF4 assOCiated factor 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SNX6 recombinant protein (Accession Number: NM_021249). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:115484(SNX6 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:115484(SNX6 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:115484(SNX6 Antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-SNX6 (IP:Catalog No:115484, 4ug; Detection:Catalog No:115484 1:600) with HepG2 cells lysate 4000ug.
1X10^6 RAW 264.7 cells were stained with 0.2ug SNX6 antibody (Catalog No:115484, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Sorting nexins (SNX) have previously been shown to regulate the cell-surface expression of the human epidermal growth factor receptor. On the basis of the predicted protein sequences, several members of this family, including SNX6, have been identified. SNX6, containing coiled-coil regions within its large C-terminal domain and is found distributed in both membrane and cytosolic fractions, typical of hydrophilic peripheral membrane proteins. The functions of SNX6 are likely to be unique to endosomes, mediated in part by interactions with SNX6-specific C-terminal sequences and membrane-associated determinants
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