-
Product Name
SNRPF antibody
- Documents
-
Description
SNRPF Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, HeLa cells, HepG2 cells, MCF7 cells, mouse kidney tissue, mouse liver tissue. Positive IHC detected in human breast cancer tissue. Positive FC detected in MCF-7 cells. Observed molecular weight by Western-blot: 10kd
-
Tested applications
ELISA, WB, IHC, FC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
PBSCF antibody; Sm F antibody; Sm protein F antibody; SMF antibody; snRNP F antibody; SNRPF antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of SNRPF recombinant protein (Accession Number: NM_003095). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
-
Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:115464(SNRPF antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115464(SNRPF antibody) at dilution of 1:100 (under 40x lens)
1X10^6 MCF-7 cells were stained with 0.2ug SNRPF antibody (Catalog No:115464, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
-
Background
The 4 major small nuclear ribonucleoprotein particles (snRNPs), U1, U2, U4/U6, and U5, are essential components of the eukaryotic pre-mRNA splicing machinery. These snRNPs share 8 proteins which form the snRNP structural core: B/B-prime (SNRPB), D1 (SNRPD1), D2 (SNRPD2), D3 (SNRPD3), E (SNRPE), F (SNRPF), and G (SNRPG). These proteins have a role in the biogenesis of the snRNPs.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"