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Product Name
SNRPA Polyclonal Antibody
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Description
Polyclonal antibody to SNRPA
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SNRPA antibody; Mud1 antibody; U1-A antibody; U1A antibody; U1 small nuclear ribonucleoprotein A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human SNRPA (NP_004587.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - SNRPA Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SNRPA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - SNRPA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human adenomyosis using SNRPA Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SNRPA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SNRPA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using SNRPA Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SNRPA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SNRPA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SNRPA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using SNRPA Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SNRPA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SNRPA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SNRPA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SNRPA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using SNRPA Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SNRPA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using SNRPA Antibody at dilution of 1:100 (40x lens).
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Background
Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"