-
Product Name
SNCG Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to SNCG
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse
-
Alternative names
SNCG antibody; BCSG1 antibody; SR antibody; gamma-synuclein antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human SNCG (NP_003078.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - SNCG Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SNCG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry - SNCG Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human thyroid cancer using SNCG antibody at dilution of 1:200 (40x lens).
Immunofluorescence - SNCG Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using SNCG antibody . Blue: DAPI for nuclear staining.
-
Background
Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"