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Product Name
SNCB antibody
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Description
SNCB Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue. Positive WB detected in mouse brain tissue, human brain tissue, rat brain tissue. Observed molecular weight by Western-blot: 14-18kd
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Beta synuclein antibody; SNCB antibody; synuclein antibody; beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SNCB recombinant protein (Accession Number: XM_006714916). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:10-1:100
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115451(SNCB antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115451(SNCB antibody) at dilution of 1:10 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using SNCB antibody Catalog No:115451 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with 0.2ug SNCB antibody (Catalog No:115451, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
SNCB represents synuclein, beta
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