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Product Name
SN-38 Monoclonal Antibody
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Description
Product has been tested in direct ELISA.
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Tested applications
ELISA
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Species reactivity
Chemical
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Isotype
Mouse IgG1, κ
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Preparation
SN-38 conjugated with BSA. Protein G affinity purified
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Clonality
Monoclonal
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Formulation
PBS(pH7.4), lyophilized. Reconstitution: Adding sterile water, prepare a stock solution of 2.0 mg/ml.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
ELISA: 1:5,000-1:20,000
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Validations
ELISA Binding Assay of Anti-SN-38 Antibody to different proteins.
ELISA analysis of Anti-SN38 Monoclonal Antibody with SN-38 -BSA, SN-38 ADC and BSA. Anti-SN-38 Monoclonal Antibody can bind to SN-38-BSA or SN-38 ADC but not carrier protein.
ELISA Binding Assay of Anti-SN-38 Antibody
Immobilized SN-38 ADC at 2μg/ /mL (100μL/well) can bind anti-SN-38 mouse monoclonal antibody.
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Background
7-Ethyl-10-hydroxycamptothecin (SN38), a highly potent metabolite of irinotecan, has an anticancer efficacy 100–1000 folds more than irinotecan in vitro. SN-38 is a topoisomerase I inhibitor and SN-38 inhibits DNA and RNA synthesis with IC50s of 0.077 and 1.3 μM, respectively.
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References
- Wallin A, Svanvik J, Holmlund B, Ferreud L, Sun XF. Anticancer effect of SN-38 on colon cancer cell lines with different metastatic potential. Oncol Rep. 2008 Jun;19(6):1493-8. PMID: 18497955.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"