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Product Name
SMYD4 antibody
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Description
SMYD4 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, mouse brain tissue. Positive IP detected in mouse brain tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas tissue, human hepat℃irrhosis tissue. Observed molecular weight by Western-blot: 89 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA1936 antibody; SMYD4 antibody; ZMYND21 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SMYD4 recombinant protein (Accession Number: XM_024450560). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115439(SMYD4 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:115439(SMYD4 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:115439(SMYD4 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-SMYD4 (IP:Catalog No:115439, 4ug; Detection:Catalog No:115439 1:500) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:115439(SMYD4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SMYD4 represents SET and MYND domain containing 4
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