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Product Name
SMURF1 antibody
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Description
SMURF1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in HEK-293 cells, HepG2 cells. Observed molecular weight by Western-blot: 83-90kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hSMURF1 antibody; KIAA1625 antibody; SMURF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_020429). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115405(SMURF1 antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:115405(SMURF1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:115405(SMURF1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. The antibody is specific to SMURF1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"