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Product Name
smooth muscle actin specific antibody
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Description
smooth muscle actin specific Rabbit Polyclonal antibody. Positive WB detected in mouse colon tissue, mouse stomach tissue. Positive IHC detected in human heart tissue, human colon cancer tissue, human colon tissue, human hepat℃irrhosis tissue, mouse colon tissue, rat colon tissue. Observed molecular weight by Western-blot: 42 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
a SMA antibody; AAT6 antibody; ACTA2 antibody; ACTA2 Smooth muscle Actin antibody; ACTA2 Smooth muscle Actin Specific antibody; Actin antibody; aortic smooth muscle antibody; Alpha actin 2 antibody; Alpha SMA antibody; Alpha SMA Specific antibody; a-SMA antibody; smooth muscle actin antibody; α SMA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001613). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human heart using Catalog No:115397(ACTA2-Specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:115397(ACTA2-Specific antibody) at dilution of 1:100 (under 40x lens)
mouse colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:115397(a-SMA specific Antibody) at dilution of 1:600
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Background
ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.
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References
- Shi H, Zhang Y, Fu S, Lu Z, Ye W, Xiao Y. Angiotensin II as a morphogenic cytokine stimulating fibrogenesis of human tenon's capsule fibroblasts. Investigative ophthalmology & visual science. 56(2):855-64. 2015.
- Dan J, Sun X, Li W. 5-Aminolevulinic Acid-Mediated Sonodynamic Therapy Promotes Phenotypic Switching from Dedifferentiated to Differentiated Phenotype via Reactive Oxygen Species and p38 Mitogen-Activated Protein Kinase in Vascular Smooth Muscle Cells. Ultrasound in medicine & biology. 41(6):1681-9. 2015.
- Zhao X, Jiang S, Liu S. Optimization of intrinsic and extrinsic tendon healing through controllable water-soluble mitomycin-C release from electrospun fibers by mediating adhesion-related gene expression. Biomaterials. 61:61-74. 2015.
- Lu L, Wang J, Lu H. MicroRNA-130a and -130b enhance activation of hepatic stellate cells by suppressing PPARγ expression: A rat fibrosis model study. Biochemical and biophysical research communications. 465(3):387-93. 2015.
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