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Product Name
SMEK2 antibody
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Description
SMEK2 Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive IF detected in HEK-293 cells. Positive WB detected in HEK-293 cells, A2780 cells, HeLa cells, MCF7 cells. Observed molecular weight by Western-blot: 94-97 kDa,87 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLFL2 antibody; FLJ31474 antibody; KIAA1387 antibody; PP4R3B antibody; PPP4R3B antibody; PSY2 antibody; SMEK homolog 2 antibody; SMEK2 antibody; smk1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SMEK2 recombinant protein (Accession Number: NM_020463). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115388(SMEK2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115388(SMEK2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115388(SMEK2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HEK-293 cells using Catalog No:115388(SMEK2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SMEK2 represents SMEK homolog 2, suppressor of mek1 (Dictyostelium)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"