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Product Name
SMEK1 Polyclonal Antibody
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Description
Polyclonal antibody to SMEK1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PPP4R3A antibody; FLFL1 antibody; KIAA2010 antibody; MSTP033 antibody; PP4R3 antibody; PP4R3A antibody; SMEK1 antibody; smk-1 antibody; smk1 antibody; protein phosphatase 4 regulatory subunit 3A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 395-594 of human SMEK1 (NP_001271210.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200 -
Validations
Western blot - SMEK1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SMEK1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - SMEK1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using SMEK1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SMEK1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using SMEK1 antibody at dilution of 1:100 (40x lens).
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Background
Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"