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Product Name
SMARCD2 antibody
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Description
SMARCD2 Rabbit Polyclonal antibody. Positive WB detected in RAW 264.7 cells, mouse thymus tissue. Positive IHC detected in human cervical cancer. Observed molecular weight by Western-blot: 52kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
SMARCD2 antibody; CRACD2 antibody; BAF60B antibody; PRO2451 antibody; Rsc6p antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SMARCD2 recombinant protein (Accession Number: BC018953). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
RAW264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:115434(SMARCD2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115434(SMARCD2 antibody) at dilution of 1:50 (under 10x lens)
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Background
The SWI/SNF chromatin remodelling complexes are important regulators of transcription; they consist of large multisubunit assemblies containing either Brm or Brg1 as the catalytic ATPase subunit and a variable subset of approximately 10 Brg/Brm-associated factors (BAF) [PMID:20148946]. SMARCD2 is one of BAF60 proteins, which are found in most complexes, is thought to bridge interactions between transcription factors and SWI/SNF complexes. [PMID:9427560]
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