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Product Name
SMARCA1 Polyclonal Antibody
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Description
Polyclonal antibody to SMARCA1
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
SMARCA1 antibody; ISWI antibody; NURF140 antibody; SNF2L antibody; SNF2L1 antibody; SNF2LB antibody; SNF2LT antibody; SWI antibody; SWI2 antibody; hSNF2L antibody; probable global transcription activator SNF2L1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SMARCA1 (NP_003060.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
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Validations
Western blot - SMARCA1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SMARCA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
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Background
Energy-transducing component of NURF (nucleosome-remodeling factor) and CERF (CECR2-containing-remodeling factor) complexes. Both complexes facilitate the perturbation of chromatin structure in an ATP-dependent manner. Potentiates neurite outgrowth. May be involved in brain development by regulating En-1 and En-2 expression. May be involved in the development of luteal cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"