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Product Name
SMAD4 antibody
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Description
SMAD4 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human heart tissue, human liver cancer tissue, human lung cancer tissue. Positive WB detected in HeLa cells, HeLa/RPE1 cells, HepG2 cells, MCF7 cells. Observed molecular weight by Western-blot: 63 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DPC4 antibody; hSMAD4 antibody; JIP antibody; MAD homolog 4 antibody; MADH4 antibody; Mothers against DPP homolog 4 antibody; SMAD 4 antibody; SMAD family member 4 antibody; SMAD4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001407042). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
WB result of Catalog No:115420.
WB result of SMAD4 antibody (Catalog No:, 1:1000) with sh-coontrol and sh-SMAD4 transfected HepG2 cells
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115420(SMAD4 antibody) at dilution of 1:1200
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115420(SMAD4 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:115420(SMAD4 antibody) at dilution of 1:50 (under 40x lens)
WB result of anti-SMAD4 (Catalog No:115420) in different cell lysates.
Immunofluorescent analysis of HepG2 cells, using SMAD4 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human SMAD4.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"