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Product Name
SMAD4 antibody
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Description
SMAD4 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IHC detected in human placenta. Observed molecular weight by Western-blot: 58kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
SMAD4 antibody; JIP antibody; SMAD family member 4 antibody; Mothers against DPP homolog 4 antibody; DPC4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001407042). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115419(SMAD4 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human placenta using Catalog No:115419(SMAD4 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:115419(SMAD4 antibody) at dilution of 1:50 (under 40x lens)
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Background
Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against a peptide (25-43aa) mapping within human SMAD4. It is specific to SMAD4.
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