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Product Name
SLC4A5 Polyclonal Antibody
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Description
Polyclonal antibody to SLC4A5
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
SLC4A5 antibody; NBC4 antibody; NBCe2 antibody; solute carrier family 4 member 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1042-1121 of human SLC4A5 (NP_597812.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - SLC4A5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SLC4A5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunohistochemistry - SLC4A5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using SLC4A5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SLC4A5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using SLC4A5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SLC4A5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using SLC4A5 antibody at dilution of 1:100 (40x lens).
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Background
Mediates sodium- and bicarbonate-dependent electrogenic sodium bicarbonate cotransport, with a Na(+):HCO3(-) stoichiometry of 2:1. May have a housekeeping function in regulating the pH of tissues in which it is expressed. May play a role in mediating Na(+):HCO3(-) cotransport in hepatocytes and intrahepatic cholangiocytes. Also may be important in protecting the renal paranchyma from alterations in urine pH.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"