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SLC38A3 antibody

Cat.#: 115374

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Special Price 239.0 USD

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Product Information

  • Product Name
    SLC38A3 antibody
  • Documents
  • Description
    SLC38A3 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive FC detected in HeLa cells. Positive WB detected in HeLa cells, BGC-823 cells, HEK-293 cells, human heart tissue, mouse brain tissue, mouse liver tissue, mouse pancreas tissue, Raji cells. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 65-70kd
  • Tested applications
    ELISA, WB, IHC, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    G17 antibody; NAT1 antibody; SLC38A3 antibody; SN1 antibody; SNAT3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SLC38A3 recombinant protein (Accession Number: XM_006712954). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115374(SLC38A3 antibody) at dilution of 1:400

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115374(SLC38A3 antibody) at dilution of 1:400

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115374(SLC38A3 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115374(SLC38A3 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115374(SLC38A3 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115374(SLC38A3 antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-SLC38A3 (IP:Catalog No:115374, 3ug; Detection:Catalog No:115374 1:1000) with mouse heart tissue lysate 4000ug.

    IP Result of anti-SLC38A3 (IP:Catalog No:115374, 3ug; Detection:Catalog No:115374 1:1000) with mouse heart tissue lysate 4000ug.

    1X10^6 HeLa cells were stained with 0.2ug SLC38A3 antibody (Catalog No:115374, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug SLC38A3 antibody (Catalog No:115374, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    SLC38A3, also known as SNAT3 or SN1, belongs to the SLC38 family of sodium-coupled neutral amino acid transporters. SLC38A3 mediates glutamine transport with sodium and proton coupled manner, and it contributes to ammonia metabolism in the liver and brain. SLC38A3 is highly expressed in the liver and brain astrocytes, with modest expression in the kidney, heart, and muscle. SLC38A3 may migrate higher than the predicted 56 kDa due to the glycosylation.
  • References
    • Frigerio F, Karaca M, De Roo M. Deletion of glutamate dehydrogenase 1 (Glud1) in the central nervous system affects glutamate handling without altering synaptic transmission. Journal of neurochemistry. 123(3):342-8. 2012.
    • Zielińska M, Dąbrowska K, Hadera MG, Sonnewald U, Albrecht J. System N transporters are critical for glutamine release and modulate metabolic fluxes of glucose and acetate in cultured cortical astrocytes: changes induced by ammonia. Journal of neurochemistry. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"