-
Product Name
SLC25A27 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to SLC25A27
-
Tested applications
WB, IF
-
Species reactivity
Mouse, Rat
-
Alternative names
SLC25A27 antibody; UCP4 antibody; mitochondrial uncoupling protein 4 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human SLC25A27 (NP_001190981.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - SLC25A27 Polyclonal Antibody
Western blot analysis of extracts of mouse brain, using SLC25A27 Antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - SLC25A27 Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using SLC25A27 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. May play a role in thermoregulatory heat production and metabolism in brain.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"