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Product Name
SLC1A2 Polyclonal Antibody
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Description
Polyclonal antibody to SLC1A2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
SLC1A2 antibody; EAAT2 antibody; EIEE41 antibody; GLT-1 antibody; HBGT antibody; solute carrier family 1 member 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 495-574 of human SLC1A2 (NP_004162.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:5000
IHC 1:100 - 1:200 -
Validations
Western blot - SLC1A2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SLC1A2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunohistochemistry - SLC1A2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using SLC1A2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SLC1A2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat pancreas using SLC1A2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SLC1A2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using SLC1A2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SLC1A2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse pancreas using SLC1A2 antibody at dilution of 1:100 (40x lens).
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Background
Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"