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Product Name
SKAP2 antibody
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Description
SKAP2 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IP detected in mouse liver tissue. Positive WB detected in human liver tissue. Observed molecular weight by Western-blot: 55kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PRAP antibody; Pyk2/RAFTK assOCiated protein antibody; RA70 antibody; SAPS antibody; SCAP2 antibody; SKAP 55HOM antibody; SKAP HOM antibody; SKAP2 antibody; SKAP55 homolog antibody; SKAP55R antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SKAP2 recombinant protein (Accession Number: XM_017012771). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:115253(SKAP2 antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115253(SKAP2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115253(SKAP2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-SKAP2 (IP:Catalog No:115253, 3ug; Detection:Catalog No:115253 1:700) with mouse liver tissue lysate 4000ug.
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Background
SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation.
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References
- Shimamura S, Sasaki K, Tanaka M. The Src substrate SKAP2 regulates actin assembly by interacting with WAVE2 and cortactin proteins. The Journal of biological chemistry. 288(2):1171-83. 2013.
- Alenghat FJ, Baca QJ, Rubin NT. Macrophages require Skap2 and Sirpα for integrin-stimulated cytoskeletal rearrangement. Journal of cell science. 125(Pt 22):5535-45. 2012.
- Ayoub E, Hall A, Scott AM. Regulation of the Src kinase-associated phosphoprotein 55 homologue by the protein tyrosine phosphatase PTP-PEST in the control of cell motility. The Journal of biological chemistry. 288(36):25739-48. 2013.
- Rolán HG, Durand EA, Mecsas J. Identifying Yersinia YopH-targeted signal transduction pathways that impair neutrophil responses during in vivo murine infection. Cell host & microbe. 14(3):306-17. 2013.
- Kuranami S, Yokobori T, Mogi A. Src kinase-associated phosphoprotein2 expression is associated with poor prognosis in non-small cell lung cancer. Anticancer research. 35(4):2411-5. 2015.
- Peng X, Zhang YY, Wang J, Ji Q. Ethylacetate extract from Tetrastigma hemsleyanum induces apoptosis via the mitochondrial caspase-dependent intrinsic pathway in HepG2 cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015.
- Zhou L, Zhang Z, Zheng Y. SKAP2, a novel target of HSF4b, associates with NCK2/F-actin at membrane ruffles and regulates actin reorganization in lens cell. Journal of cellular and molecular medicine. 15(4):783-95. 2011.
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