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Product Name
SIX4 antibody
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Description
SIX4 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 98 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AREC3 antibody; Homeobox protein SIX4 antibody; Sine OCulis homeobox homolog 4 antibody; SIX homeobox 4 antibody; SIX4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SIX4 recombinant protein (Accession Number: BC098282). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115251(SIX4 antibody) at dilution of 1:300
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:115251(SIX4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The Six (sine oculis) proteins are a family of homeodomain transcription factors that share a conserved DNA-binding domain and are human homologs of the Drosophila sine oculis (so) protein. SIX homeobox 4 (SIX4), also known as AREC3, belongs to the the Six family. Six4 contains one Six domain (which funtions as a homeobox DNA-binding motif) and shares 90% sequence similarity with its mouse counterpart, suggesting that both proteins have similar DNA-binding properties. SIX4 is thought to be involved in neurogenesis, myogenesis, and development of other organs, based on its specific expression in certain neuronal cells of the developing embryo and in adult skeletal muscles.
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