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SIX2 antibody

Cat.#: 115250

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Product Information

  • Product Name
    SIX2 antibody
  • Documents
  • Description
    SIX2 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human renal cell carcinoma tissue. Positive IF detected in mouse embryonic kidney, heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice. Positive WB detected in HEK-293 cells, L02 cells, PC-3 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 37-40kd
  • Tested applications
    ELISA, IHC, IF, IP, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Homeobox protein SIX2 antibody; Sine OCulis homeobox homolog 2 antibody; SIX homeobox 2 antibody; SIX2 antibody
  • Immunogen
    Human SIX2 Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SIX2 recombinant protein (Accession Number: NM_016932). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115250(SIX2 Antibody) at dilution of 1:600

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115250(SIX2 Antibody) at dilution of 1:600

    IP Result of anti-SIX2 (IP:Catalog No:115250, 3ug; Detection:Catalog No:115250 1:500) with HEK-293 cells lysate 2700ug.

    IP Result of anti-SIX2 (IP:Catalog No:115250, 3ug; Detection:Catalog No:115250 1:500) with HEK-293 cells lysate 2700ug.

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:115250(SIX2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:115250(SIX2 antibody) at dilution of 1:50 (under 10x lens)

    IF result of SIX2 antibody (Catalog No:115250, 1:200) with mouse embryonic kidney rudiment dissected at E13.5 and cultured for 2 days by Dr. Aleksandra Rak-Raszewska. SIX2 positive cells (red) in condense metanephric mesenchyme surrounding the ureteric bud tip.

    IF result of SIX2 antibody (Catalog No:115250, 1:200) with mouse embryonic kidney rudiment dissected at E13.5 and cultured for 2 days by Dr. Aleksandra Rak-Raszewska. SIX2 positive cells (red) in condense metanephric mesenchyme surrounding the ureteric bud tip.

    IF result from Fogelgren B, PMID:18570229,

    IF result from Fogelgren B, PMID:18570229,

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:115250(SIX2 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:115250(SIX2 antibody) at dilution of 1:50 (under 40x lens)

  • Background
    The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.
  • References
    • lyu Z, Mao Z, Wang H. MiR-181b targets Six2 and inhibits the proliferation of metanephric mesenchymal cells in vitro. Biochemical and biophysical research communications. 440(4):495-501. 2013.
    • Murphy AJ, Pierce J, de Caestecker C. Aberrant activation, nuclear localization, and phosphorylation of Yes-associated protein-1 in the embryonic kidney and Wilms tumor. Pediatric blood & cancer. 61(2):198-205. 2014.
    • Xia Y, Nivet E, Sancho-Martinez I. Directed differentiation of human pluripotent cells to ureteric bud kidney progenitor-like cells. Nature cell biology. 15(12):1507-15. 2013.
    • Takasato M, Er PX, Becroft M. Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney. Nature cell biology. 16(1):118-26. 2014.
    • Hilliard SA, Yao X, El-Dahr SS. Mdm2 is required for maintenance of the nephrogenic niche. Developmental biology. 387(1):1-14. 2014.
    • Hum S, Rymer C, Schaefer C, Bushnell D, Sims-Lucas S. Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning. PloS one. 9(2):e88400. 2014.
    • Ahn SY, Kim Y, Kim ST, Swat W, Miner JH. Scaffolding proteins DLG1 and CASK cooperate to maintain the nephron progenitor population during kidney development. Journal of the American Society of Nephrology : JASN. 24(7):1127-38. 2013.
    • Zhou P, Chen T, Fang Y. Down-regulated Six2 by knockdown of neurofibromin results in apoptosis of metanephric mesenchyme cells in vitro. Molecular and cellular biochemistry. 390(1-2):205-13. 2014.

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