-
Product Name
SIM2 antibody
- Documents
-
Description
SIM2 Rabbit Polyclonal antibody. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in COLO 320 cells, human skeletal muscle tissue, mouse skeletal muscle tissue, PC-3 cells. Observed molecular weight by Western-blot: 60-64 kDa
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
bHLHe15 antibody; SIM antibody; SIM2 antibody; Single minded homolog 2 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of SIM2 recombinant protein (Accession Number: NM_009586). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
-
Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:115233(SIM2 antibody) at dilution of 1:1000
IP Result of anti-SIM2 (IP:Catalog No:115233, 4ug; Detection:Catalog No:115233 1:800) with mouse skeletal muscle tissue lysate 2200ug.
-
Background
SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"