-
Product Name
SIK1 antibody
- Documents
-
Description
SIK1 Rabbit Polyclonal antibody. Positive WB detected in A549 cells, Jurkat cells. Positive IHC detected in human lung cancer tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 85kd
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
MSK antibody; salt inducible kinase 1 antibody; SIK antibody; SIK 1 antibody; SIK1 antibody; SNF1LK antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of SIK1 recombinant protein (Accession Number: NM_173354). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:115230(SIK1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115230(SIK1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115230(SIK1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using Sik1 antibody Catalog No:115230 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
-
Background
SIK1(Salt-inducible kinase 1) is also named as SIK, SNF1LK and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for phosphorylation of class II HDACs and for expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancers. Loss of SIK1 correlates with poor patient outcome in breast cancers(PMID:19622832).
-
References
- Cheng J, Uchida M, Zhang W, Grafe MR, Herson PS, Hurn PD. Role of salt-induced kinase 1 in androgen neuroprotection against cerebral ischemia. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 31(1):339-50. 2011.
- Lombardi MS, Gilliéron C, Dietrich D, Gabay C. SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype. Journal of leukocyte biology. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"