SIINFEKL peptide (TFA removed)

SIINFEKL peptide (TFA removed)

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-SIINFEKL-OH

H-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu-OH

8

98.23%

C₄₅H₇₄N₁₀O₁₃

963.12

Synthetic

Powder

SIINFEKL is a peptide derived from ovalbumin (ova) and has been extensively studied for its application in understanding antigen processing and presentation by antigen-presenting cells (APCs) such as dendritic cells. SIINFEKL-specific OT1 T cells can recognize and respond to this peptide, making it a valuable tool for measuring antigen-specific CD8+ T cell responses in vivo. Vaccination with ova-SIINFEKL peptide conjugates has been shown to elicit a robust immune response, leading to increased proliferation of OT1 T cells and production of IFNg, a cytokine associated with T cell activation and effector function. The use of SIINFEKL peptide in combination with other immune stimulators, such as polyI:C, has demonstrated enhanced antigen-specific T cell responses, highlighting its potential as a component in vaccine development. Overall, SIINFEKL peptide offers a valuable tool for studying immune responses and has potential applications in vaccine design and immunotherap

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Otto M, Süssmuth R, Jung G, Götz F. Structure of the pheromone peptide of the Staphylococcus epidermidis agr system. FEBS Lett. 1998 Mar 6;424(1-2):89-94. doi:10.1016/s0014-5793(98)00145-8. PMID:9537521.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.