Human STAT1 (His & GST Tag) recombinant protein

Human STAT1 (His & GST Tag) recombinant protein

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Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Signal transducer and activator of transcription 1-alpha/beta

DKFZP686B04100; ISGF-3; IMD31B; STAT1; CANDF7; IMD31C; IMD31A; AA408197; STAT91; 2010005J02RIK

P42224

STAT1

Baculovirus-Insect Cells

A DNA sequence encoding the human STAT1 isoform beta (P42224-2) (Met 1-Val 712) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human STAT1/GST chimera consists of 950 amino acids and has a calculated molecular mass of 111 kDa. It migrates as an approximately 105 kDa band in SDS-PAGE under reducing conditions.

> 92 % as determined by SDS-PAGE