-
Product Name
SIGLEC12 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to SIGLEC12
-
Tested applications
WB
-
Species reactivity
Mouse
-
Alternative names
SIGLEC12 antibody; S2V antibody; SIGLECL1 antibody; SLG antibody; Siglec-XII antibody; sialic acid-binding Ig-like lectin 12 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-210 of human SIGLEC12 (NP_443729.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - SIGLEC12 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SIGLEC12 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
-
Background
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"