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Product Name
SIAH1 antibody
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Description
SIAH1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A549 cells, COLO 320 cells, HepG2 cells, Jurkat cells, mouse small intestine tissue, RAW 264.7 cells. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB
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Species reactivity
human, mouse; other species not tested.
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Alternative names
SIAH1 antibody; hSIAH1 antibody; Siah 1a antibody; Seven in absentia homolog 1 antibody; Siah 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SIAH1 recombinant protein (Accession Number: XM_024450395). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115284(SIAH1 antibody) at dilution of 1:300
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Background
SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)
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