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Product Name
SHMT2 antibody
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Description
SHMT2 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human cervical cancer tissue, human heart tissue. Positive WB detected in HeLa cells, HEK-293 cells, MCF7 cells, mouse kidney tissue. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
GLYA antibody; Serine methylase antibody; SHMT antibody; SHMT2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SHMT2 recombinant protein (Accession Number: NM_005412). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115277(SHMT2 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115277(SHMT2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:115277(SHMT2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SHMT2 represents serine hydroxymethyltransferase 2 (mitochondrial)
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References
- Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"