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Product Name
SHC3 antibody
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Description
SHC3 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in BxPC-3 cells. Positive IP detected in BxPC-3 cells. Observed molecular weight by Western-blot: 64 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
N Shc antibody; Neuronal Shc antibody; NSHC antibody; Protein Rai antibody; RAI antibody; SH2 domain protein C3 antibody; SHC transforming protein 3 antibody; SHC transforming protein C antibody; SHC3 antibody; SHCC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SHC3 recombinant protein (Accession Number: NM_016848). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115267(SHC3 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:115267(SHC3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:115267(SHC3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-SHC3 (IP:Catalog No:115267, 4ug; Detection:Catalog No:115267 1:300) with BxPC-3 cells lysate 2800ug.
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Background
SHC3 represents SHC (Src homology 2 domain containing) transforming protein 3
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References
- Sevecka M, Wolf-Yadlin A, MacBeath G. Lysate microarrays enable high-throughput, quantitative investigations of cellular signaling. Molecular & cellular proteomics : MCP. 10(4):M110.005363. 2011.
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