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Product Name
SHC antibody
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Description
SHC Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, HeLa cells. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 52kd and 66kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ26504 antibody; SH2 domain protein C1 antibody; SHC antibody; SHC transforming protein 1 antibody; SHC transforming protein 3 antibody; SHC transforming protein A antibody; SHC1 antibody; SHCA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SHC recombinant protein (Accession Number: XM_047427974). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115265(SHC1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:115265(SHC1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using SHC1 antibody Catalog No:115265 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
SHC1 represents SHC (Src homology 2 domain containing) transforming protein 1
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