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Product Name
SH3GL1 Polyclonal Antibody
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Description
Polyclonal antibody to SH3GL1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat, Monkey
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Alternative names
SH3GL1 antibody; CNSA1 antibody; EEN antibody; SH3D2B antibody; SH3P8 antibody; endophilin-A2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 219-368 of human SH3GL1 (NP_003016.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200 -
Validations
Western blot - SH3GL1 Polyclonal Antibody
Western blot analysis of extracts of COS-7 cells, using SH3GL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
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Background
Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"