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Product Name
SH3BP5 antibody
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Description
SH3BP5 Rabbit Polyclonal antibody. Positive WB detected in Raji cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 47kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
SAB antibody; SH3 domain binding protein 5 antibody; SH3BP 5 antibody; SH3BP5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SH3BP5 recombinant protein (Accession Number: NM_004844). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:115190(SH3BP5 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:115190(SH3BP5 antibody) at dilution of 1:50 (under 10x lens)
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Background
SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detecte the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.
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References
- Win S, Than TA, Fernandez-Checa JC, Kaplowitz N. JNK interaction with Sab mediates ER stress induced inhibition of mitochondrial respiration and cell death. Cell death & disease. 5:e989. 2014.
- Win S, Than TA, Le BH, García-Ruiz C, Fernandez-Checa JC, Kaplowitz N. Sab (Sh3bp5) dependence of JNK mediated inhibition of mitochondrial respiration in palmitic acid induced hepatocyte lipotoxicity. Journal of hepatology. 62(6):1367-74. 2015.
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