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Product Name
SGSM3 antibody
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Description
SGSM3 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human spleen tissue. Positive IF detected in Hela cells. Positive WB detected in mouse brain tissue, L02 cells, mouse testis tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 85 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp761D051 antibody; MAP antibody; Merlin assOCiated protein antibody; RabGAPLP antibody; RUSC3 antibody; RUTBC3 antibody; SGSM3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SGSM3 recombinant protein (Accession Number: NM_015705). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115175(SGSM3 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human heart using Catalog No:115175(SGSM3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:115175(SGSM3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using SGSM3 antibody Catalog No:115175 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-SGSM3 (IP:Catalog No:115175, 4ug; Detection:Catalog No:115175 1:600) with mouse brain tissue lysate 3440ug.
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Background
SGSM3 represents small G protein signaling modulator 3
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