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Product Name
SGCE Polyclonal Antibody
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Description
Polyclonal antibody to SGCE
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
SGCE antibody; DYT11 antibody; ESG antibody; epsilon-SG antibody; epsilon-sarcoglycan antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human SGCE (NP_003910.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - SGCE Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SGCE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - SGCE Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using SGCE antibody at dilution of 1:200 (40x lens).
Immunofluorescence - SGCE Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using SGCE antibody . Blue: DAPI for nuclear staining.
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Background
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"