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Product Name
SFTPB Polyclonal Antibody
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Description
Polyclonal antibody to SFTPB
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
SFTPB antibody; PSP-B antibody; SFTB3 antibody; SFTP3 antibody; SMDP1 antibody; SP-B antibody; surfactant protein B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 94-393 of human SFTPB (NP_000533.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - SFTPB Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SFTPB Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.
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Background
Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"