Cart 2

SFRP4 antibody

Cat.#: 115146

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    SFRP4 antibody
  • Documents
  • Description
    SFRP4 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells. Positive IP detected in HeLa cells. Positive IHC detected in human cervical cancer tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 41-51 kDa; 55 kDa
  • Tested applications
    ELISA, WB, IP, IHC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    FRP 4 antibody; FRPHE antibody; sFRP 4 antibody; SFRP4 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SFRP4 recombinant protein (Accession Number: NM_003014). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:50-1:500

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115146(SFRP4 antibody) at dilution of 1:400

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115146(SFRP4 antibody) at dilution of 1:400

    IP Result of anti-SFRP4 (IP:Catalog No:115146, 4ug; Detection:Catalog No:115146 1:500) with HeLa cells lysate 2000ug.

    IP Result of anti-SFRP4 (IP:Catalog No:115146, 4ug; Detection:Catalog No:115146 1:500) with HeLa cells lysate 2000ug.

    Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:115146(SFRP4 Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:115146(SFRP4 Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:115146(SFRP4 Antibody) at dilution of 1:200 (under 40x lens).

    Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:115146(SFRP4 Antibody) at dilution of 1:200 (under 40x lens).

  • Background
    Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP4 functions as a suppressor of tumor growth via inhibition of the Wnt signaling pathway.Downregulation or deletion of SFRP4 has been reported in several cancers and cell lines such as endometrial cancer, ovarian cancer, lung cancer,and others. This antibody got 41-51 kDa and 55 kDa bands in western blotting maybe due to differential posttranslational protein modification.
  • References
    • Mortensen MM, Høyer S, Lynnerup AS. Expression profiling of prostate cancer tissue delineates genes associated with recurrence after prostatectomy. Scientific reports. 5:16018. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"