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Product Name
SF4 antibody
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Description
SF4 Rabbit Polyclonal antibody. Positive IF detected in HepG2. Positive IHC detected in human testis. Positive WB detected in HEK-293 cells, HepG2 cells, mouse testis tissue. Observed molecular weight by Western-blot: 72kd,55kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
SF4 antibody; DKFZp434E2216 antibody; RBP antibody; F23858 antibody; RNA binding protein RBP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SF4 recombinant protein (Accession Number: BC002986). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115141(SF4 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:115141(SF4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:115141(SF4 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using SF4 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
SF4 represents splicing factor 4
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"