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Product Name
SF3B4 antibody
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Description
SF3B4 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells, PC-3 cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
SAP 49 antibody; SAP49 antibody; SF3B4 antibody; SF3b49 antibody; SF3b50 antibody; Splicing factor 3B subunit 4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SF3B4 recombinant protein (Accession Number: NM_005850). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115139(SF3B4 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:115139(SF3B4 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-SF3B4 (IP:Catalog No:115139, 4ug; Detection:Catalog No:115139 1:500) with HeLa cells lysate 1600ug.
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Background
SF3B4 represents splicing factor 3b, subunit 4, 49kDa
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