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Product Name
SF3B2 Polyclonal Antibody
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Description
Polyclonal antibody to SF3B2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SF3B2 antibody; Cus1 antibody; SAP145 antibody; SF3B145 antibody; SF3b1 antibody; SF3b150 antibody; splicing factor 3B subunit 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 616-895 of human SF3B2 (NP_006833.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - SF3B2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SF3B2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - SF3B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SF3B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SF3B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SF3B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SF3B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SF3B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human oophoroma using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SF3B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney cancer using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SF3B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SF3B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse Intestine using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SF3B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SF3B2 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using SF3B2 antibody .
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Background
Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
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